Unravelling the Therapeutic Potential of Antibiotics in Hypoxia in a Breast Cancer MCF-7 Cell Line Model

Antibiotics inhibit breast cancer stem cells (CSCs) by suppressing mitochondrial biogenesis. However, the effectiveness of antibiotics in clinical settings is inconsistent. This inconsistency raises question whether tumor microenvironment, particularly hypoxia, plays a role response to antibiotics. Therefore, goal this study was evaluate five commonly used for inhibiting CSCs under hypoxia using an MCF-7 cell line model. We assessed number through mammosphere formation assay and aldehyde dehydrogenase (ALDH)-bright count. Additionally, we examined impact on stress membrane potential. Furthermore, analyzed levels proteins associated with therapeutic resistance. There no significant difference between cultured normoxic hypoxic conditions. did affect rate CSC inhibition Specifically, azithromycin unable sphere hypoxia. Erythromycin doxycycline not reduce ratio ALDH-bright cells, despite decreasing mammospheres. treatment chloramphenicol, doxycycline, tetracycline led overexpression resistance protein. Our findings suggest that may weaken inhibitory effects